Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2- (2-methoxy-4-nitrophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2H- tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-methoxy PMS.
CCK-8 solution is added directly to cells, no pre-mixing of components is required. WST-8 is reduced by cellular dehydrogenases to a formazan orange product that is soluble in a tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and has low cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 enables sensitive colourimetric assays for the determination of the number of viable cells in proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salt like MTT, TXT or MTS.
Properties
- Quality level 100
- limited shelf life, the expiration date on the label
- sent in environment
- storage temperature. 2-8 ° C
