Przydatność kliniczna cyfrowej PCR kropli do monitorowania transkryptów

Przydatność kliniczna cyfrowej PCR kropli do monitorowania transkryptów

Przydatność kliniczna cyfrowej PCR kropli do monitorowania transkryptów BCR-ABL1 pacjentów z ostrą białaczką limfoblastyczną z dodatnim chromosomem Philadelphia Put up-chimeryczny receptor antygenowy 19/22 T-Cell Cocktail Remedy

 

Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) accounts for 20-30% of grownup sufferers with ALL, characterised by translocation of t (9, 22). Tyrosine kinase inhibitors (TKIs) have considerably improved the result although there are nonetheless some issues together with relapse as a result of drug-resistant mutations and suboptimal molecular remission depth. Beforehand, we reported the security and efficacy of sequential infusion of CD19/22 chimeric antigen receptor T-cell (CAR-T) immunotherapy within the therapy of relapsed/refractory (R/R) B-cell neoplasms together with circumstances with Ph+ ALL. Given doable deeper response, extra sufferers have been anticipated to achieve optimum minimal residual illness (MRD) response.

An alternate technique, duplex droplet digital PCR (ddPCR) with excessive sensitivity was established, which may present absolute quantification of MRD with out the necessity for calibration curves. Right here, we retrospectively collected 95 bone marrow samples from 10 sufferers with R/R Ph+, who acquired 19/22 CAR-T-cell cocktail remedy. Notably, sequential molecular remission for greater than three months (SMR3), a major indicator primarily based on ddPCR after CAR-T infusion was established, which was outlined as a sequential molecular remission for not <three months with damaging MRD. On this cohort, no recurrence was noticed in six sufferers reaching SMR3, the place 4 of whom accepted allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CAR-T cell routine.

Sadly, the opposite 4 sufferers who didn’t attain SMR3 relapsed, and didn’t obtain further particular therapy besides CAR-T routine. To sum up, ddPCR could also be another, particularly when nucleic acid was inadequate in medical apply. No achievement of SMR3 could be an early warning of potential relapse after CAR-T and indicating the initiation of different therapies together with allo-HSCT.

 

predictad
predictad

Anti-CD207 antibody (Alexa-fluor 647)

STJ170016 100 µg
EUR 471.6
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 647)

STJ170022 100 µg
EUR 471.6
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

SAM FCM (Alexa Fluor 647)

abx098902-100tests 100 tests
EUR 1479.6

Anti-Alexa Fluor 647 Antibody | M647-65A-400

M647-65A-400 1.0 mg
EUR 379
Description:

Anti-Alexa Fluor 647 Antibody | M647-65A-400 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

iFluor® 647 Styramide *Superior Replacement for Alexa Fluor 647 tyramide*

45045 100 Slides
EUR 334

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)

43R-ID028AF 500 ug
EUR 516
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)

43R-ID060AF 300 ug
EUR 510
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647

A6-686-C100 0.1 mg
EUR 322.8

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

A6-712-C100 0.1 mg
EUR 322.8

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647

A6-730-C100 0.1 mg
EUR 322.8

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647

A6-737-C100 0.1 mg
EUR 322.8

XFD647 maleimide *C2, XFD647 Same Structure to Alexa Fluor™ 647*

1895 1 mg
EUR 222

GPR12 Antibody

44910-100ul 100ul
EUR 302.4

GPR12 Antibody

44910-50ul 50ul
EUR 224.4

GPR12 antibody

70R-49804 100 ul
EUR 292.8
Description: Purified Polyclonal GPR12 antibody

GPR12 antibody

70R-30940 100 ug
EUR 392.4
Description: Rabbit polyclonal GPR12 antibody

GPR12 antibody

20R-GR041 50 ug
EUR 787.2
Description: Rabbit polyclonal GPR12 antibody

GPR12 Antibody

ABD2721 100 ug
EUR 525.6

GPR12 Antibody

DF2721 200ul
EUR 420

GPR12 Antibody

1-CSB-PA008905
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000

GPR12 Antibody

1-CSB-PA009742LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

EGFR antibody (Alexa Fluor 488)

61R-E109BAF 125 ug
EUR 847.2
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)

GPR12

RA30050 50 ug
EUR 741.6

XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*

1833 1 mg
EUR 222

XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*

71909 5 mg
EUR 781

Endoglin/CD105 Alexa Fluor

FC15024 100 Tests
EUR 537.6

XFD647 Anti-human CD47 Antibody *HI172, XFD647 Same Structure to Alexa Fluor™ 647*

10471180 100 tests
EUR 245

XFD647 Anti-human CD47 Antibody *HI172, XFD647 Same Structure to Alexa Fluor™ 647*

10471181 500 tests
EUR 918

XFD647 Anti-human CD52 Antibody *HI186, XFD647 Same Structure to Alexa Fluor™ 647*

10520180 100 tests
EUR 245

XFD647 Anti-human CD52 Antibody *HI186, XFD647 Same Structure to Alexa Fluor™ 647*

10520181 500 tests
EUR 918

XFD647 Anti-human CD53 Antibody *HI29, XFD647 Same Structure to Alexa Fluor™ 647*

10530180 100 tests
EUR 245

XFD647 Anti-human CD53 Antibody *HI29, XFD647 Same Structure to Alexa Fluor™ 647*

10530181 500 tests
EUR 918

XFD647 Anti-human CD53 Antibody *HI36, XFD647 Same Structure to Alexa Fluor™ 647*

10531180 100 tests
EUR 245

XFD647 Anti-human CD53 Antibody *HI36, XFD647 Same Structure to Alexa Fluor™ 647*

10531181 500 tests
EUR 918

XFD647 Anti-human CD55 Antibody *HI55a, XFD647 Same Structure to Alexa Fluor™ 647*

10550180 100 tests
EUR 245

XFD647 Anti-human CD55 Antibody *HI55a, XFD647 Same Structure to Alexa Fluor™ 647*

10550181 500 tests
EUR 918

XFD647 Anti-human CD56 Antibody *My31, XFD647 Same Structure to Alexa Fluor™ 647*

10562170 100 tests
EUR 245

XFD647 Anti-human CD56 Antibody *My31, XFD647 Same Structure to Alexa Fluor™ 647*

10562171 500 tests
EUR 918

XFD647 Anti-human CD57 Antibody *HI57a, XFD647 Same Structure to Alexa Fluor™ 647*

10570180 100 tests
EUR 245

XFD647 Anti-human CD57 Antibody *HI57a, XFD647 Same Structure to Alexa Fluor™ 647*

10570181 500 tests
EUR 918

XFD647 Anti-human CD58 Antibody *HI58a, XFD647 Same Structure to Alexa Fluor™ 647*

10580180 100 tests
EUR 245

XFD647 Anti-human CD58 Antibody *HI58a, XFD647 Same Structure to Alexa Fluor™ 647*

10580181 500 tests
EUR 918

XFD647 Anti-human CD62 Antibody *HI62E, XFD647 Same Structure to Alexa Fluor™ 647*

10620180 100 tests
EUR 245

XFD647 Anti-human CD62 Antibody *HI62E, XFD647 Same Structure to Alexa Fluor™ 647*

10620181 500 tests
EUR 918

XFD647 Anti-human CD62l Antibody *HI62L, XFD647 Same Structure to Alexa Fluor™ 647*

10621180 100 tests
EUR 245

XFD647 Anti-human CD62l Antibody *HI62L, XFD647 Same Structure to Alexa Fluor™ 647*

10621181 500 tests
EUR 918

XFD647 Anti-human CD62p Antibody *HI62P, XFD647 Same Structure to Alexa Fluor™ 647*

10622180 100 tests
EUR 245

XFD647 Anti-human CD62p Antibody *HI62P, XFD647 Same Structure to Alexa Fluor™ 647*

10622181 500 tests
EUR 918

XFD647 Anti-human CD64 Antibody *10.1, XFD647 Same Structure to Alexa Fluor™ 647*

10640180 100 tests
EUR 245

XFD647 Anti-human CD64 Antibody *10.1, XFD647 Same Structure to Alexa Fluor™ 647*

10640181 500 tests
EUR 918

XFD647 Anti-human CD69 Antibody *FN50, XFD647 Same Structure to Alexa Fluor™ 647*

10690180 100 tests
EUR 547

XFD647 Anti-human CD69 Antibody *FN50, XFD647 Same Structure to Alexa Fluor™ 647*

10690181 500 tests
EUR 2051

XFD647 Anti-human CD71 Antibody *HI160, XFD647 Same Structure to Alexa Fluor™ 647*

10710180 100 tests
EUR 245

XFD647 Anti-human CD71 Antibody *HI160, XFD647 Same Structure to Alexa Fluor™ 647*

10710181 500 tests
EUR 918

XFD647 Anti-human CD71 Antibody *HI166, XFD647 Same Structure to Alexa Fluor™ 647*

10711180 100 tests
EUR 245

XFD647 Anti-human CD71 Antibody *HI166, XFD647 Same Structure to Alexa Fluor™ 647*

10711181 500 tests
EUR 918

XFD647 Anti-human CD72 Antibody *3F3, XFD647 Same Structure to Alexa Fluor™ 647*

10720180 100 tests
EUR 547

XFD647 Anti-human CD72 Antibody *3F3, XFD647 Same Structure to Alexa Fluor™ 647*

10720181 500 tests
EUR 2051

XFD647 Anti-human CD73 Antibody *AD2, XFD647 Same Structure to Alexa Fluor™ 647*

10730180 100 tests
EUR 547

XFD647 Anti-human CD73 Antibody *AD2, XFD647 Same Structure to Alexa Fluor™ 647*

10730181 500 tests
EUR 2051

XFD647 Anti-human CD5 Antibody *HISM2, XFD647 Same Structure to Alexa Fluor™ 647*

10050180 100 tests
EUR 245

XFD647 Anti-human CD5 Antibody *HISM2, XFD647 Same Structure to Alexa Fluor™ 647*

10050181 500 tests
EUR 918

XFD647 Anti-human CD5 Antibody *L17F12, XFD647 Same Structure to Alexa Fluor™ 647*

10051180 100 tests
EUR 245

XFD647 Anti-human CD5 Antibody *L17F12, XFD647 Same Structure to Alexa Fluor™ 647*

10051181 500 tests
EUR 918

XFD647 Anti-human CD5 Antibody *UCHT2, XFD647 Same Structure to Alexa Fluor™ 647*

10052180 100 tests
EUR 245

XFD647 Anti-human CD5 Antibody *UCHT2, XFD647 Same Structure to Alexa Fluor™ 647*

10052181 500 tests
EUR 918

XFD647 Anti-human CD6 Antibody *HI210, XFD647 Same Structure to Alexa Fluor™ 647*

10060180 100 tests
EUR 245

XFD647 Anti-human CD6 Antibody *HI210, XFD647 Same Structure to Alexa Fluor™ 647*

10060181 500 tests
EUR 918

XFD647 Anti-human CD7 Antibody *HIT7, XFD647 Same Structure to Alexa Fluor™ 647*

10070180 100 tests
EUR 245

XFD647 Anti-human CD7 Antibody *HIT7, XFD647 Same Structure to Alexa Fluor™ 647*

10070181 500 tests
EUR 918

XFD647 Anti-human CD8 Antibody *HIT8a, XFD647 Same Structure to Alexa Fluor™ 647*

10080180 100 tests
EUR 245

XFD647 Anti-human CD8 Antibody *HIT8a, XFD647 Same Structure to Alexa Fluor™ 647*

10080181 500 tests
EUR 918

XFD647 Anti-human CD8 Antibody *SK1, XFD647 Same Structure to Alexa Fluor™ 647*

10081180 100 tests
EUR 245

XFD647 Anti-human CD8 Antibody *SK1, XFD647 Same Structure to Alexa Fluor™ 647*

10081181 500 tests
EUR 918

XFD647 Anti-human CD9 Antibody *HI9a, XFD647 Same Structure to Alexa Fluor™ 647*

10090180 100 tests
EUR 245

XFD647 Anti-human CD9 Antibody *HI9a, XFD647 Same Structure to Alexa Fluor™ 647*

10090181 500 tests
EUR 918

XFD647 Anti-human CD10 Antibody *HI10a, XFD647 Same Structure to Alexa Fluor™ 647*

10100180 100 tests
EUR 245

XFD647 Anti-human CD10 Antibody *HI10a, XFD647 Same Structure to Alexa Fluor™ 647*

10100181 500 tests
EUR 918

XFD647 Anti-human CD11a Antibody *HI111, XFD647 Same Structure to Alexa Fluor™ 647*

10110180 100 tests
EUR 245

XFD647 Anti-human CD11a Antibody *HI111, XFD647 Same Structure to Alexa Fluor™ 647*

10110181 500 tests
EUR 918

XFD647 Anti-human CD11b Antibody *HI11b, XFD647 Same Structure to Alexa Fluor™ 647*

10111180 100 tests
EUR 245

XFD647 Anti-human CD11b Antibody *HI11b, XFD647 Same Structure to Alexa Fluor™ 647*

10111181 500 tests
EUR 918

XFD647 Anti-human CD11b Antibody *ICRF44, XFD647 Same Structure to Alexa Fluor™ 647*

10112180 100 tests
EUR 245

XFD647 Anti-human CD11b Antibody *ICRF44, XFD647 Same Structure to Alexa Fluor™ 647*

10112181 500 tests
EUR 918

XFD647 Anti-human CD11c Antibody *3.9, XFD647 Same Structure to Alexa Fluor™ 647*

10113180 100 tests
EUR 245

XFD647 Anti-human CD11c Antibody *3.9, XFD647 Same Structure to Alexa Fluor™ 647*

10113181 500 tests
EUR 918

XFD647 Anti-human CD13 Antibody *WM15, XFD647 Same Structure to Alexa Fluor™ 647*

10130180 100 tests
EUR 245

XFD647 Anti-human CD13 Antibody *WM15, XFD647 Same Structure to Alexa Fluor™ 647*

10130181 500 tests
EUR 918

XFD647 Anti-human CD14 Antibody *61D3, XFD647 Same Structure to Alexa Fluor™ 647*

10141180 100 tests
EUR 245

XFD647 Anti-human CD14 Antibody *61D3, XFD647 Same Structure to Alexa Fluor™ 647*

10141181 500 tests
EUR 918

XFD647 Anti-human CD15 Antibody *HI98, XFD647 Same Structure to Alexa Fluor™ 647*

10150180 100 tests
EUR 296

XFD647 Anti-human CD15 Antibody *HI98, XFD647 Same Structure to Alexa Fluor™ 647*

10150181 500 tests
EUR 1020

XFD647 Anti-human CD16 Antibody *HI16a, XFD647 Same Structure to Alexa Fluor™ 647*

10160180 100 tests
EUR 245

XFD647 Anti-human CD16 Antibody *HI16a, XFD647 Same Structure to Alexa Fluor™ 647*

10160181 500 tests
EUR 918

XFD647 Anti-human CD16 Antibody *3G8, XFD647 Same Structure to Alexa Fluor™ 647*

10161180 100 tests
EUR 245

XFD647 Anti-human CD16 Antibody *3G8, XFD647 Same Structure to Alexa Fluor™ 647*

10161181 500 tests
EUR 918

XFD647 Anti-human CD18 Antibody *HI18a, XFD647 Same Structure to Alexa Fluor™ 647*

10180180 100 tests
EUR 245

XFD647 Anti-human CD18 Antibody *HI18a, XFD647 Same Structure to Alexa Fluor™ 647*

10180181 500 tests
EUR 918

XFD647 Anti-human CD19 Antibody *HI19a, XFD647 Same Structure to Alexa Fluor™ 647*

10190180 100 tests
EUR 245

XFD647 Anti-human CD19 Antibody *HI19a, XFD647 Same Structure to Alexa Fluor™ 647*

10190181 500 tests
EUR 918

XFD647 Anti-human CD19 Antibody *SJ25C1, XFD647 Same Structure to Alexa Fluor™ 647*

10191180 100 tests
EUR 245

XFD647 Anti-human CD19 Antibody *SJ25C1, XFD647 Same Structure to Alexa Fluor™ 647*

10191181 500 tests
EUR 918

XFD647 Anti-human CD19 Antibody *HIB19, XFD647 Same Structure to Alexa Fluor™ 647*

10192180 100 tests
EUR 245

XFD647 Anti-human CD19 Antibody *HIB19, XFD647 Same Structure to Alexa Fluor™ 647*

10192181 500 tests
EUR 918

XFD647 Anti-human CD19 Antibody *4G7, XFD647 Same Structure to Alexa Fluor™ 647*

10193180 100 tests
EUR 245

XFD647 Anti-human CD19 Antibody *4G7, XFD647 Same Structure to Alexa Fluor™ 647*

10193181 500 tests
EUR 918

XFD647 Anti-mouse CD19 Antibody *1D3, XFD647 Same Structure to Alexa Fluor™ 647*

10194170 100 tests
EUR 245

XFD647 Anti-mouse CD19 Antibody *1D3, XFD647 Same Structure to Alexa Fluor™ 647*

10194171 500 tests
EUR 918

XFD647 Anti-human CD20 Antibody *HI20a, XFD647 Same Structure to Alexa Fluor™ 647*

10201180 100 tests
EUR 245

XFD647 Anti-human CD20 Antibody *HI20a, XFD647 Same Structure to Alexa Fluor™ 647*

10201181 500 tests
EUR 918

XFD647 Anti-human CD21 Antibody *HI21a, XFD647 Same Structure to Alexa Fluor™ 647*

10210180 100 tests
EUR 245

XFD647 Anti-human CD21 Antibody *HI21a, XFD647 Same Structure to Alexa Fluor™ 647*

10210181 500 tests
EUR 918

XFD647 Anti-human CD22 Antibody *HIB22, XFD647 Same Structure to Alexa Fluor™ 647*

10220180 100 tests
EUR 245

XFD647 Anti-human CD22 Antibody *HIB22, XFD647 Same Structure to Alexa Fluor™ 647*

10220181 500 tests
EUR 918

XFD647 Anti-human CD101 Antibody *BB27, XFD647 Same Structure to Alexa Fluor™ 647*

11010170 100 tests
EUR 547

XFD647 Anti-human CD101 Antibody *BB27, XFD647 Same Structure to Alexa Fluor™ 647*

11010171 500 tests
EUR 2051

XFD647 Anti-human CD107 Antibody *H4B4, XFD647 Same Structure to Alexa Fluor™ 647*

11071170 100 tests
EUR 547

XFD647 Anti-human CD107 Antibody *H4B4, XFD647 Same Structure to Alexa Fluor™ 647*

11071171 500 tests
EUR 2051

XFD647 Anti-human CD109 Antibody *W7C5, XFD647 Same Structure to Alexa Fluor™ 647*

11090170 100 tests
EUR 547

XFD647 Anti-human CD109 Antibody *W7C5, XFD647 Same Structure to Alexa Fluor™ 647*

11090171 500 tests
EUR 2051

XFD647 Anti-human CD111 Antibody *R1.302, XFD647 Same Structure to Alexa Fluor™ 647*

11110170 100 tests
EUR 547

XFD647 Anti-human CD111 Antibody *R1.302, XFD647 Same Structure to Alexa Fluor™ 647*

11110171 500 tests
EUR 2051

XFD647 Anti-human CD112 Antibody *R2.525, XFD647 Same Structure to Alexa Fluor™ 647*

11120170 100 tests
EUR 547

XFD647 Anti-human CD112 Antibody *R2.525, XFD647 Same Structure to Alexa Fluor™ 647*

11120171 500 tests
EUR 2051

XFD647 Anti-human CD114 Antibody *LMM741, XFD647 Same Structure to Alexa Fluor™ 647*

11140170 100 tests
EUR 547

XFD647 Anti-human CD114 Antibody *LMM741, XFD647 Same Structure to Alexa Fluor™ 647*

11140171 500 tests
EUR 2051

XFD647 Anti-human CD116 Antibody *4H1, XFD647 Same Structure to Alexa Fluor™ 647*

11160170 100 tests
EUR 547

XFD647 Anti-human CD116 Antibody *4H1, XFD647 Same Structure to Alexa Fluor™ 647*

11160171 500 tests
EUR 2051

XFD647 Anti-human CD117 Antibody *104D2, XFD647 Same Structure to Alexa Fluor™ 647*

11170180 100 tests
EUR 547

XFD647 Anti-human CD117 Antibody *104D2, XFD647 Same Structure to Alexa Fluor™ 647*

11170181 500 tests
EUR 2051

XFD647 Anti-human CD120a Antibody *H398, XFD647 Same Structure to Alexa Fluor™ 647*

11200170 100 tests
EUR 547

XFD647 Anti-human CD120a Antibody *H398, XFD647 Same Structure to Alexa Fluor™ 647*

11200171 500 tests
EUR 2051

XFD647 Anti-human CD122 Antibody *TU27, XFD647 Same Structure to Alexa Fluor™ 647*

11220170 100 tests
EUR 547

XFD647 Anti-human CD122 Antibody *TU27, XFD647 Same Structure to Alexa Fluor™ 647*

11220171 500 tests
EUR 2051

XFD647 Anti-human CD123 Antibody *6H6, XFD647 Same Structure to Alexa Fluor™ 647*

11230180 100 tests
EUR 157

XFD647 Anti-human CD123 Antibody *6H6, XFD647 Same Structure to Alexa Fluor™ 647*

11230181 500 tests
EUR 484

XFD647 Anti-human CD123 Antibody *12H7, XFD647 Same Structure to Alexa Fluor™ 647*

11231170 100 tests
EUR 245

XFD647 Anti-human CD123 Antibody *12H7, XFD647 Same Structure to Alexa Fluor™ 647*

11231171 500 tests
EUR 918

XFD647 Anti-human CD133 Antibody *293C3, XFD647 Same Structure to Alexa Fluor™ 647*

11330170 100 tests
EUR 607

XFD647 Anti-human CD133 Antibody *293C3, XFD647 Same Structure to Alexa Fluor™ 647*

11330171 500 tests
EUR 2280

XFD647 Anti-human CD135 Antibody *BV10A4, XFD647 Same Structure to Alexa Fluor™ 647*

11350170 100 tests
EUR 547

XFD647 Anti-human CD135 Antibody *BV10A4, XFD647 Same Structure to Alexa Fluor™ 647*

11350171 500 tests
EUR 2051

XFD647 Anti-human CD138 Antibody *MI15, XFD647 Same Structure to Alexa Fluor™ 647*

11380180 100 tests
EUR 245

XFD647 Anti-human CD138 Antibody *MI15, XFD647 Same Structure to Alexa Fluor™ 647*

11380181 500 tests
EUR 918

XFD647 Anti-human CD140a Antibody *16A1, XFD647 Same Structure to Alexa Fluor™ 647*

11400170 100 tests
EUR 547

XFD647 Anti-human CD140a Antibody *16A1, XFD647 Same Structure to Alexa Fluor™ 647*

11400171 500 tests
EUR 2051

XFD647 Anti-human CD140b Antibody *18A2, XFD647 Same Structure to Alexa Fluor™ 647*

11401170 100 tests
EUR 547

XFD647 Anti-human CD140b Antibody *18A2, XFD647 Same Structure to Alexa Fluor™ 647*

11401171 500 tests
EUR 2051

XFD647 Anti-human CD24 Antibody *HI45, XFD647 Same Structure to Alexa Fluor™ 647*

10240180 100 tests
EUR 245

XFD647 Anti-human CD24 Antibody *HI45, XFD647 Same Structure to Alexa Fluor™ 647*

10240181 500 tests
EUR 918

XFD647 Anti-human CD25 Antibody *HI25a, XFD647 Same Structure to Alexa Fluor™ 647*

10250180 100 tests
EUR 245

XFD647 Anti-human CD25 Antibody *HI25a, XFD647 Same Structure to Alexa Fluor™ 647*

10250181 500 tests
EUR 918

XFD647 Anti-human CD25 Antibody *7G7B6, XFD647 Same Structure to Alexa Fluor™ 647*

10251180 100 tests
EUR 245

XFD647 Anti-human CD25 Antibody *7G7B6, XFD647 Same Structure to Alexa Fluor™ 647*

10251181 500 tests
EUR 918

XFD647 Anti-human CD27 Antibody *LT27, XFD647 Same Structure to Alexa Fluor™ 647*

10270180 100 tests
EUR 547

XFD647 Anti-human CD27 Antibody *LT27, XFD647 Same Structure to Alexa Fluor™ 647*

10270181 500 tests
EUR 2051

XFD647 Anti-human CD27 Antibody *O323, XFD647 Same Structure to Alexa Fluor™ 647*

10271170 100 tests
EUR 245

XFD647 Anti-human CD27 Antibody *O323, XFD647 Same Structure to Alexa Fluor™ 647*

10271171 500 tests
EUR 918

XFD647 Anti-human CD28 Antibody *CD28.2, XFD647 Same Structure to Alexa Fluor™ 647*

10280180 100 tests
EUR 245

XFD647 Anti-human CD28 Antibody *CD28.2, XFD647 Same Structure to Alexa Fluor™ 647*

10280181 500 tests
EUR 918

XFD647 Anti-human CD28 Antibody *9.3, XFD647 Same Structure to Alexa Fluor™ 647*

10281180 100 tests
EUR 245

XFD647 Anti-human CD28 Antibody *9.3, XFD647 Same Structure to Alexa Fluor™ 647*

10281181 500 tests
EUR 918

XFD647 Anti-human CD29 Antibody *HI29a, XFD647 Same Structure to Alexa Fluor™ 647*

10290180 100 tests
EUR 245

XFD647 Anti-human CD29 Antibody *HI29a, XFD647 Same Structure to Alexa Fluor™ 647*

10290181 500 tests
EUR 918

XFD647 Anti-human CD32 Antibody *3D3, XFD647 Same Structure to Alexa Fluor™ 647*

10321180 100 tests
EUR 547

Ilościowe wykrywanie i monitorowanie Colletotrichum siamense na drzewach kauczukowych przy użyciu PCR w czasie rzeczywistym

Colletotrichum siamense is without doubt one of the most necessary pathogens of rubber timber in Asia. The right detection and quantification of C. siamense populations in rubber timber are of significance for monitoring the epidemics of the illness. On this research, we developed an ITS-based real-time PCR technique to effectively detect C. siamense infecting rubber tree, which reliably detected as little as 100 fg genomic DNA, 100 copies of goal DNA and 20 conidia.

The actual-time PCR protocol acknowledged all C. siamense isolates collected from three provinces in China, whereas no amplification was noticed with rubber tree and its different pathogens. Detection and quantification of C. siamense have been carried out in artificially and naturally contaminated rubber leaves. We may nonetheless detect C. siamense in plant mixes of which solely 0.0001% of the tissue contaminated. An accumulation of C. siamense DNA was noticed throughout the entire an infection course of in any respect three leaf phenological levels, suggesting the real-time PCR technique can be utilized to watch C. siamense improvement in rubber timber. Lastly, the tactic allowed the detection of C. siamense in naturally contaminated and symptomless leaves of rubber timber within the fields. In contrast with earlier detection strategies, the real-time PCR technique is extra particular and extra delicate, and might be of nice use for research aiming to achieve a greater understanding of the epidemiology of Colletotrichum leaf illness, in addition to the prediction of illness danger and the management proposal.

Ilościowy pomiar liczby kopii transpozonu przy użyciu cyfrowej PCR kropli

Spontaneous proliferation of transposable parts contributes to genetic range at various ranges reminiscent of somatic mosaicism, genetic divergence in inhabitants, and genome evolution. Such genetic range is important for vegetation’ adaptation to altering atmosphere and serves as a useful useful resource for crop enchancment. Subsequently, measuring the copy quantity variation of transposable parts with precision and effectivity is necessary to grasp the extent of their proliferation.

Droplet Digital PCR (ddPCR) is an correct and delicate method that permits measurement of copy quantity variation of a transposon. Briefly, genomic DNA is extracted, digested, and partitioned into 1000’s of nanoliter-scale droplets. The TaqMan real-time PCR adopted by the end-point fluorescence detection allows the quantitative measurement of copy variety of template DNAs. Right here on this chapter, we describe the step-by-step process of ddPCR utilizing EVADE retrotransposon of Arabidopsis for example.

 

Porównanie wyników tomografii komputerowej (CT) z RT-PCR w diagnostyce COVID-19 u dzieci

 

polymerase chain response (RT-PCR) check ends in kids with possible or definitive prognosis of coronavirus illness 2019 (COVID-19).

Strategies: On this retrospective archive research, pediatric sufferers who have been adopted up within the hospital with a doable or definitive prognosis of COVID-19 and who had chest CT at presentation have been included. CT scan photos of the sufferers have been reinterpreted by a pediatric radiologist and in contrast with their RT-PCR check outcomes.

Outcomes: Of the whole of 89 sufferers, 33 had damaging and 56 had constructive RT-PCR exams. The presence of pulmonary lesions and consolidation was statistically considerably greater within the RT-PCR damaging group than within the RT-PCR constructive group (p = 0.037 and 0.001, respectively). Lobe involvement of 0%-25% was greater within the RT-PCR constructive group (p = 0.001), and lobe involvements of 25%-50% and 50%-75% have been considerably greater within the RT-PCR damaging group (p = 0.001 and 0.005, respectively). Central and perihilar involvement was discovered to be statistically important in the RT-PCR damaging group (p = 0.008 and 0.005, respectively).

Conclusion: Chest CT findings could present some clues in predicting RT-PCR positivity in kids with a possible prognosis of COVID-19. Lobe involvement proportion of as much as 25% is a discovering in favor of sufferers with constructive RT-PCR check, whereas 25%-75% lobe involvement, central and perihilar involvement, and consolidation might be interpreted in favor of sufferers with damaging RT-PCR check.

 

Integracja przygotowania próbek mikroprzepływowych z detekcją PCR w celu zbadania skutków jednoczesnej separacji inhibitora DNA i wymiany roztworu DNA

 

On this paper, we utilized a curved-channel microfluidic system to separate DNA from PCR-inhibitor-containing water and concurrently wash them into clear water for detection utilizing a conveyable PCR thermocycler. Environmental DNA (eDNA) sampling has turn into an efficient surveying method for detecting uncommon organisms. Nonetheless, low focus eDNA molecules could also be masked by PCR inhibitors throughout amplification and detection, growing the chance of false negatives. Subsequently, applied sciences for on-site DNA separation and washing are urgently wanted. Our system consisted of a half-circle microchannel with a DNA-inhibitor pattern inlet, a clear buffer inlet, and a number of retailers. Through the use of the flow-induced inertial forces, 10 μm DNA-conjugated microparticles have been targeted on the inner-wall of the curved microchannel whereas separation from 1 μm inhibitor-conjugated microparticles and DNA washing have been achieved concurrently with the Dean circulation.

We achieved singleplex focusing, isolation and washing of 10 μm particles at an effectivity of 94.5 ± 2.0%. In duplex experiments with 1 μm and 10 μm particles, bigger particles have been washed with an effectivity of 92.1 ± 1.6% and a purity of 79 ± 2%. By surface-functionalizing the microparticles with affinity teams towards Atlantic salmon DNA and humic acid (HA), and processing samples of varied concentrations in our system, we achieved an efficient purification and detection of DNA molecules utilizing the transportable PCR thermocycler. Our technique considerably decreased PCR quantitation cycles from Cq > 38 to Cq = 30.35 ± 0.5, which confirmed enhancement of PCR amplification. The proposed system takes a promising step ahead in pattern preparation in the direction of an built-in system that can be utilized for simultaneous purification and resolution alternate of DNA in point-of-need environmental monitoring functions.

Share

Leave a Reply

Your email address will not be published. Required fields are marked *