Przydatność kliniczna cyfrowej PCR kropli do monitorowania transkryptów

Przydatność kliniczna cyfrowej PCR kropli do monitorowania transkryptów

Przydatność kliniczna cyfrowej PCR kropli do monitorowania transkryptów BCR-ABL1 pacjentów z ostrą białaczką limfoblastyczną z dodatnim chromosomem Philadelphia Put up-chimeryczny receptor antygenowy 19/22 T-Cell Cocktail Remedy

 

Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) accounts for 20-30% of grownup sufferers with ALL, characterised by translocation of t (9, 22). Tyrosine kinase inhibitors (TKIs) have considerably improved the result although there are nonetheless some issues together with relapse as a result of drug-resistant mutations and suboptimal molecular remission depth. Beforehand, we reported the security and efficacy of sequential infusion of CD19/22 chimeric antigen receptor T-cell (CAR-T) immunotherapy within the therapy of relapsed/refractory (R/R) B-cell neoplasms together with circumstances with Ph+ ALL. Given doable deeper response, extra sufferers have been anticipated to achieve optimum minimal residual illness (MRD) response.

An alternate technique, duplex droplet digital PCR (ddPCR) with excessive sensitivity was established, which may present absolute quantification of MRD with out the necessity for calibration curves. Right here, we retrospectively collected 95 bone marrow samples from 10 sufferers with R/R Ph+, who acquired 19/22 CAR-T-cell cocktail remedy. Notably, sequential molecular remission for greater than three months (SMR3), a major indicator primarily based on ddPCR after CAR-T infusion was established, which was outlined as a sequential molecular remission for not <three months with damaging MRD. On this cohort, no recurrence was noticed in six sufferers reaching SMR3, the place 4 of whom accepted allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CAR-T cell routine.

Sadly, the opposite 4 sufferers who didn’t attain SMR3 relapsed, and didn’t obtain further particular therapy besides CAR-T routine. To sum up, ddPCR could also be another, particularly when nucleic acid was inadequate in medical apply. No achievement of SMR3 could be an early warning of potential relapse after CAR-T and indicating the initiation of different therapies together with allo-HSCT.

 

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Anti-LAMP3 antibody (Alexa-fluor 647)

STJ170006 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 647)

STJ170011 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 647)

STJ170016 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 647)

STJ170022 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Donkey anti Goat IgG (H + L) (Alexa Fluor 647)

43R-ID028AF 500 ug
EUR 430
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 647)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 647)

43R-ID060AF 300 ug
EUR 425
Description: Donkey anti Chicken IgY (H + L) (Fab'2) (Alexa Fluor 647)

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 647–conjugated

S0013 200ul
EUR 304

Goat Anti-Mouse IgG(H+L) Alexa Fluor 647–conjugated

S0014 200ul
EUR 304

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 647

A6-686-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 647

A6-712-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 647

A6-730-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 647

A6-737-C100 0.1 mg
EUR 269

EGFR antibody (Alexa Fluor 488)

61R-E109BAF 125 ug
EUR 706
Description: Mouse monoclonal EGFR antibody (Alexa Fluor 488)

Endoglin/CD105 Alexa Fluor

FC15024 100 Tests
EUR 448

Anti-CD40 antibody (Alexa-fluor 488)

STJ170000 100 µg
EUR 393
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin

Anti-LAMP3 antibody (Alexa-fluor 488)

STJ170004 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 546)

STJ170005 100 µg
EUR 393
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)

STJ170009 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 546)

STJ170010 100 µg
EUR 393
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)

STJ170014 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 546)

STJ170015 100 µg
EUR 393
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)

STJ170020 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 546)

STJ170021 100 µg
EUR 393
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

SAM FCM (Alexa Fluor 488)

abx098904-60tests 60 tests
EUR 1358
  • Shipped within 5-10 working days.

Anti-RPSA Alexa Fluor® 488

A4-829-C100 0.1 mg
EUR 310

Mouse IgG1-Alexa 647 conjugate (isotype control)

20102-101-A647 50 Tests
EUR 263

Goat anti Mouse IgG1 (Alexa Fluor 488)

43R-1649 500 ug
EUR 570
Description: Goat anti Mouse IgG1 secondary antibody (Alexa Fluor 488)

Anti-Hu CD16 Alexa Fluor® 488

A4-646-T100 100 tests
EUR 269

Alpha Fluor™ 532 acid [equivalent to Alexa Fluor™ 532 acid]

1795 10 mg
EUR 393
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF350 Phalloidin [equivalent to Alexa Fluor® 350 phalloidin]

23150 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

AF488 Phalloidin [equivalent to Alexa Fluor® 488 phalloidin]

23153 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

23158 300 Tests
EUR 306
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

AF350-streptavidin conjugate [Streptavidin, Alexa Fluor™ 350 Conjugate]

16890 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF488-streptavidin conjugate [Streptavidin, Alexa Fluor™ 488 Conjugate]

16891 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

16892 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF 500 ug
EUR 338
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF 500 ug
EUR 364
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF 500 ug
EUR 462
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-ID056AF 500 ug
EUR 343
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-IR016AF 1 mg
EUR 281
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

Anti-Hu CD72 Alexa Fluor® 488

A4-310-T100 100 tests
EUR 269

Anti-Bov CD9 Alexa Fluor® 488

A4-354-C100 0.1 mg
EUR 269

Anti-Hu CD30 Alexa Fluor® 700

A7-455-T100 100 tests
EUR 269

Anti-Hu CD94 Alexa Fluor® 700

A7-727-T100 100 tests
EUR 269

Anti-Hu CD56 Alexa Fluor® 700

A7-789-T100 100 tests
EUR 269

GPR12 antibody

20R-GR041 50 ug
EUR 656
Description: Rabbit polyclonal GPR12 antibody

GPR12 antibody

70R-30940 100 ug
EUR 327
Description: Rabbit polyclonal GPR12 antibody

GPR12 Antibody

44910-100ul 100ul
EUR 252

GPR12 Antibody

44910-50ul 50ul
EUR 187

GPR12 Antibody

DF2721 200ul
EUR 304
Description: GPR12 antibody detects endogenous levels of total GPR12.

GPR12 antibody

70R-49804 100 ul
EUR 244
Description: Purified Polyclonal GPR12 antibody

GPR12 Antibody

1-CSB-PA008905
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/5000

GPR12 Antibody

1-CSB-PA009742LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

GPR12 Antibody

ABD2721 100 ug
EUR 438

Goat Anti-Mouse IgG(H+L) Alexa Fluor 594–conjugated

S0005 200ul
EUR 376

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 594–conjugated

S0006 200ul
EUR 376

Goat Anti-Mouse IgG(H+L) Alexa Fluor 488–conjugated

S0017 200ul
EUR 304

Goat Anti-Rabbit IgG(H+L) Alexa Fluor 488–conjugated

S0018 200ul
EUR 304

Anti-LAMP3 (human) Monoclonal Antibody (104G4) (Alexa Fluor® 488)

M09406 100ug
EUR 565
Description: Mouse Monoclonal LAMP3 (human) Antibody (104G4) (Alexa Fluor® 488). Validated in IHC and tested in Human.

GPR12

RA30050 50 ug
EUR 618

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)

43R-ID012AF 300 ug
EUR 410
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

Ab8011-001 1mg
EUR 334

Donkey Anti-Rabbit IgG (H+L), Alexa Fluor® 488 Conjugated

Ab8032-001 0.5mg
EUR 435

Anti-Hu CD3 zeta (pY153) Alexa Fluor® 488

A4-686-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY72) Alexa Fluor® 488

A4-712-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY142) Alexa Fluor® 488

A4-730-C100 0.1 mg
EUR 269

Anti-Hu CD3 zeta (pY111) Alexa Fluor® 488

A4-737-C100 0.1 mg
EUR 269

Gpr12/ Rat Gpr12 ELISA Kit

ELI-09764r 96 Tests
EUR 886

GPR12 Conjugated Antibody

C44910 100ul
EUR 397

GPR12 Polyclonal Antibody

ABP54586-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ABP54586-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of GPR12 from Human. This GPR12 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the N-terminal region of human GPR12 at AA rangle: 10-90

GPR12 Polyclonal Antibody

ES5585-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

GPR12 Polyclonal Antibody

ES5585-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against GPR12 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA

Anti-GPR12 antibody

STJ93328 200 µl
EUR 197
Description: Rabbit polyclonal to GPR12.

Anti-Langerin (human) Monoclonal Antibody (DCGM4/122D5) (Alexa Fluor® 488)

M02316 100ug
EUR 580
Description: Mouse Monoclonal Langerin (human) Antibody (DCGM4/122D5) (Alexa Fluor® 488). Validated in IHC and tested in Human.

XL-647

B2204-1
EUR 142

XL-647

B2204-5
EUR 403

GPR12 siRNA

20-abx902268
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 siRNA

20-abx918427
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

GPR12 siRNA

20-abx918428
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

Anti-GPR12/Gpcr12 Antibody

A10818 100ul
EUR 397
Description: Rabbit Polyclonal GPR12/Gpcr12 Antibody. Validated in IF and tested in Human.

GPR12 Antibody, HRP conjugated

1-CSB-PA009742LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GPR12 Antibody, FITC conjugated

1-CSB-PA009742LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GPR12 Antibody, Biotin conjugated

1-CSB-PA009742LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against GPR12. Recognizes GPR12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

gp100 (639–647)

5-01247 4 x 5mg Ask for price

Cyanine 647 aminooxy

96008 1MG
EUR 342
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 647 alkyne

92101 1MG
EUR 226
Description: Minimum order quantity: 1 unit of 1MG

Rabbit Anti-Rat IgG (H+L)-Alexa 488 Fluor conjugate (adsorbed with human IgG)

50336 0.5 ml
EUR 225

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)

50337 0.5 ml
EUR 225

GPR12 Blocking Peptide

DF2721-BP 1mg
EUR 195

GPR12 Blocking Peptide

20-abx062679
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg
  • Shipped within 5-10 working days.

Polyclonal GPR12 Antibody (Extracellular Domain)

APR16480G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (Extracellular Domain). This antibody is tested and proven to work in the following applications:

Polyclonal GPR12 Antibody (N-Terminus)

APR16481G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 (N-Terminus). This antibody is tested and proven to work in the following applications:

iFluor™ 647 amine

1074 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Cyanine 647 succinimidyl ester

9-90025
  • EUR 1100.00
  • EUR 226.00
  • 10MG
  • 1MG
Description: Minimum order quantity: 1 unit of 1MG

gp100 (639-647) Peptide

20-abx266103
  • EUR 411.00
  • EUR 662.00
  • EUR 314.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.

Annexin V Dyomics 647

EXB0023 100 tests
EUR 222

hsa-miR-647 Primers

MPH01949 150 ul / 10 uM
EUR 121

Anti-Irs4 (mouse aa633-647) antibody

STJ72906 100 µg
EUR 359

Monoclonal Anti-Monkey IgG-Alexa 647 Conj. (specific for monkey; no reactivity with human or animals IgG)

70030-AF647 50 tests
EUR 347

GPR12 ELISA KIT|Human

EF005024 96 Tests
EUR 689

Rat GPR12 shRNA Plasmid

20-abx986567
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Human GPR12 shRNA Plasmid

20-abx951900
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Mouse GPR12 shRNA Plasmid

20-abx970621
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

GPR12 Recombinant Protein (Human)

RP061035 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203285 100 ug Ask for price

GPR12 Recombinant Protein (Rat)

RP203288 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139421 100 ug Ask for price

GPR12 Recombinant Protein (Mouse)

RP139424 100 ug Ask for price

Polyclonal GPR12 Antibody - C-terminal region

APR16482G 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR12 - C-terminal region. This antibody is tested and proven to work in the following applications:

Phalloidin-iFluor™ 647 Conjugate

23127 300 Tests
EUR 176
  • R-phrase: R23, R24, R25
  • H-Phrase: H301, H311, H331
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200

iFluor™ 647-streptavidin conjugate

16966 200 ug
EUR 115
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

iFluor™ 647-streptavidin conjugate

16996 1 mg
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Cyanine 647-dUTP, lyophilized powder

40065 25nmol
EUR 236
Description: Minimum order quantity: 1 unit of 25nmol

hsa-miR-647 miRNA Inhibitor

MIH03303 2 x 5.0 nmol
EUR 176

hsa-miR-647 miRNA Antagomir

MNH03303 2 x 5.0 nmol
EUR 329

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx009208
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx147559
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx324375
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody

20-abx338465
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Gpr12 ORF Vector (Rat) (pORF)

ORF067763 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Rat) (pORF)

ORF067764 1.0 ug DNA
EUR 506

GPR12 ORF Vector (Human) (pORF)

ORF020346 1.0 ug DNA
EUR 405

Gpr12 ORF Vector (Mouse) (pORF)

ORF046475 1.0 ug DNA
EUR 506

Gpr12 ORF Vector (Mouse) (pORF)

ORF046476 1.0 ug DNA
EUR 506

Alpha Fluor™ 488 amine

1705 1 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Alpha Fluor™ 488 Hydroxylamine

1900 1 mg
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Tide Fluor 2-LL-37

H-8286.0100 0.1mg
EUR 312
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 2-LL-37

H-8286.0500 0.5mg
EUR 1017
Description: Sum Formula: C205H340N60O53+dye

Anti-Cytokeratins Alexa Fluor488

A4-108-C025 0.025 mg
EUR 175

Anti-Cytokeratins Alexa Fluor488

A4-108-C100 0.1 mg
EUR 310

Anti-PSMA Alexa Fluor488

A4-539-C025 0.025 mg
EUR 227

Anti-PSMA Alexa Fluor488

A4-539-C100 0.1 mg
EUR 414

Anti-FoxP3 Alexa Fluor488

A4-601-C025 0.025 mg
EUR 201

Anti-FoxP3 Alexa Fluor488

A4-601-C100 0.1 mg
EUR 362

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C025 0.025 mg
EUR 154

Anti-Phosphotyrosine Alexa Fluor647

A6-263-C100 0.1 mg
EUR 269

Anti-LCK Alexa Fluor647

A6-269-C025 0.025 mg
EUR 206

Anti-LCK Alexa Fluor647

A6-269-C100 0.1 mg
EUR 373

Anti-FoxP3 Alexa Fluor647

A6-601-C025 0.025 mg
EUR 201

Anti-FoxP3 Alexa Fluor647

A6-601-C100 0.1 mg
EUR 362

Annexin V-iFluor™ 647 conjugate

20074 100 tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

ViaFluor 647 Live Cell Microtubule Stain

70063 50uL
EUR 439
Description: Minimum order quantity: 1 unit of 50uL

RPE-iFluor™ 647-streptavidin conjugate

16906 100 ug
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Recombinant Hemagglutinin Influenza A Virus H3N2 Wisconsin 67/05

RP-647 2 ug
EUR 164

G Protein Coupled Receptor 12 (GPR12) Antibody (HRP)

20-abx335850
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody (FITC)

20-abx335851
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

G Protein Coupled Receptor 12 (GPR12) Antibody (Biotin)

20-abx335852
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-10 working days.

Gpr12 sgRNA CRISPR Lentivector set (Rat)

K7010601 3 x 1.0 ug
EUR 339

Gpr12 sgRNA CRISPR Lentivector set (Mouse)

K3382801 3 x 1.0 ug
EUR 339

GPR12 sgRNA CRISPR Lentivector set (Human)

K0889201 3 x 1.0 ug
EUR 339

Rabbit Anti-Human PRKCD (aa 643-647) Polyclonal Antibody

CPB-1170RH 100 ul
EUR 460

Rabbit Anti-Human STAT6 Polyclonal Antibody, aa.643-647

CPB-963RH 100 ul
EUR 460

Metal Fluor™ Zn-520, AM

21263 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Alpha Fluor™ 488 NHS Ester

1812 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Alpha Fluor™ 532 NHS Ester

1819 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Alpha Fluor™ 594 C5 Maleimide

1891 1 mg
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Helix Fluor™ 6-JOE Phosphoramidite

6046 100 umoles
EUR 50
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Tide Fluor 2-LL-37 (scrambled)

H-8288.0100 0.1mg
EUR 312
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 2-LL-37 (scrambled)

H-8288.0500 0.5mg
EUR 1017
Description: Sum Formula: C205H340N60O53+dye

Tide Fluor 5WS-o-Conotoxin GVIA

H-8356.0100 0.1mg
EUR 1146
Description: Sum Formula: C120H182N38O43S6+dye

Streptavidin-Alexa488 (Alexas fluor 488) conjugate

SV-A488-100 100 tests
EUR 225

Streptavidin-Alexa594 (Alexas fluor 594) conjugate

SV-A594-100 100 tests
EUR 225

Anti-Cytokeratin 18 Alexa Fluor488

A4-106-C025 0.025 mg
EUR 186

Ilościowe wykrywanie i monitorowanie Colletotrichum siamense na drzewach kauczukowych przy użyciu PCR w czasie rzeczywistym

Colletotrichum siamense is without doubt one of the most necessary pathogens of rubber timber in Asia. The right detection and quantification of C. siamense populations in rubber timber are of significance for monitoring the epidemics of the illness. On this research, we developed an ITS-based real-time PCR technique to effectively detect C. siamense infecting rubber tree, which reliably detected as little as 100 fg genomic DNA, 100 copies of goal DNA and 20 conidia.

The actual-time PCR protocol acknowledged all C. siamense isolates collected from three provinces in China, whereas no amplification was noticed with rubber tree and its different pathogens. Detection and quantification of C. siamense have been carried out in artificially and naturally contaminated rubber leaves. We may nonetheless detect C. siamense in plant mixes of which solely 0.0001% of the tissue contaminated. An accumulation of C. siamense DNA was noticed throughout the entire an infection course of in any respect three leaf phenological levels, suggesting the real-time PCR technique can be utilized to watch C. siamense improvement in rubber timber. Lastly, the tactic allowed the detection of C. siamense in naturally contaminated and symptomless leaves of rubber timber within the fields. In contrast with earlier detection strategies, the real-time PCR technique is extra particular and extra delicate, and might be of nice use for research aiming to achieve a greater understanding of the epidemiology of Colletotrichum leaf illness, in addition to the prediction of illness danger and the management proposal.

Ilościowy pomiar liczby kopii transpozonu przy użyciu cyfrowej PCR kropli

Spontaneous proliferation of transposable parts contributes to genetic range at various ranges reminiscent of somatic mosaicism, genetic divergence in inhabitants, and genome evolution. Such genetic range is important for vegetation’ adaptation to altering atmosphere and serves as a useful useful resource for crop enchancment. Subsequently, measuring the copy quantity variation of transposable parts with precision and effectivity is necessary to grasp the extent of their proliferation.

Droplet Digital PCR (ddPCR) is an correct and delicate method that permits measurement of copy quantity variation of a transposon. Briefly, genomic DNA is extracted, digested, and partitioned into 1000’s of nanoliter-scale droplets. The TaqMan real-time PCR adopted by the end-point fluorescence detection allows the quantitative measurement of copy variety of template DNAs. Right here on this chapter, we describe the step-by-step process of ddPCR utilizing EVADE retrotransposon of Arabidopsis for example.

 

Porównanie wyników tomografii komputerowej (CT) z RT-PCR w diagnostyce COVID-19 u dzieci

 

polymerase chain response (RT-PCR) check ends in kids with possible or definitive prognosis of coronavirus illness 2019 (COVID-19).

Strategies: On this retrospective archive research, pediatric sufferers who have been adopted up within the hospital with a doable or definitive prognosis of COVID-19 and who had chest CT at presentation have been included. CT scan photos of the sufferers have been reinterpreted by a pediatric radiologist and in contrast with their RT-PCR check outcomes.

Outcomes: Of the whole of 89 sufferers, 33 had damaging and 56 had constructive RT-PCR exams. The presence of pulmonary lesions and consolidation was statistically considerably greater within the RT-PCR damaging group than within the RT-PCR constructive group (p = 0.037 and 0.001, respectively). Lobe involvement of 0%-25% was greater within the RT-PCR constructive group (p = 0.001), and lobe involvements of 25%-50% and 50%-75% have been considerably greater within the RT-PCR damaging group (p = 0.001 and 0.005, respectively). Central and perihilar involvement was discovered to be statistically important in the RT-PCR damaging group (p = 0.008 and 0.005, respectively).

Conclusion: Chest CT findings could present some clues in predicting RT-PCR positivity in kids with a possible prognosis of COVID-19. Lobe involvement proportion of as much as 25% is a discovering in favor of sufferers with constructive RT-PCR check, whereas 25%-75% lobe involvement, central and perihilar involvement, and consolidation might be interpreted in favor of sufferers with damaging RT-PCR check.

 

Integracja przygotowania próbek mikroprzepływowych z detekcją PCR w celu zbadania skutków jednoczesnej separacji inhibitora DNA i wymiany roztworu DNA

 

On this paper, we utilized a curved-channel microfluidic system to separate DNA from PCR-inhibitor-containing water and concurrently wash them into clear water for detection utilizing a conveyable PCR thermocycler. Environmental DNA (eDNA) sampling has turn into an efficient surveying method for detecting uncommon organisms. Nonetheless, low focus eDNA molecules could also be masked by PCR inhibitors throughout amplification and detection, growing the chance of false negatives. Subsequently, applied sciences for on-site DNA separation and washing are urgently wanted. Our system consisted of a half-circle microchannel with a DNA-inhibitor pattern inlet, a clear buffer inlet, and a number of retailers. Through the use of the flow-induced inertial forces, 10 μm DNA-conjugated microparticles have been targeted on the inner-wall of the curved microchannel whereas separation from 1 μm inhibitor-conjugated microparticles and DNA washing have been achieved concurrently with the Dean circulation.

We achieved singleplex focusing, isolation and washing of 10 μm particles at an effectivity of 94.5 ± 2.0%. In duplex experiments with 1 μm and 10 μm particles, bigger particles have been washed with an effectivity of 92.1 ± 1.6% and a purity of 79 ± 2%. By surface-functionalizing the microparticles with affinity teams towards Atlantic salmon DNA and humic acid (HA), and processing samples of varied concentrations in our system, we achieved an efficient purification and detection of DNA molecules utilizing the transportable PCR thermocycler. Our technique considerably decreased PCR quantitation cycles from Cq > 38 to Cq = 30.35 ± 0.5, which confirmed enhancement of PCR amplification. The proposed system takes a promising step ahead in pattern preparation in the direction of an built-in system that can be utilized for simultaneous purification and resolution alternate of DNA in point-of-need environmental monitoring functions.

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