Przydatność kliniczna cyfrowej PCR kropli do monitorowania transkryptów BCR-ABL1 pacjentów z ostrą białaczką limfoblastyczną z dodatnim chromosomem Philadelphia Put up-chimeryczny receptor antygenowy 19/22 T-Cell Cocktail Remedy
Philadelphia chromosome-positive acute lymphoblastic leukemia (Ph+ ALL) accounts for 20-30% of grownup sufferers with ALL, characterised by translocation of t (9, 22). Tyrosine kinase inhibitors (TKIs) have considerably improved the result although there are nonetheless some issues together with relapse as a result of drug-resistant mutations and suboptimal molecular remission depth. Beforehand, we reported the security and efficacy of sequential infusion of CD19/22 chimeric antigen receptor T-cell (CAR-T) immunotherapy within the therapy of relapsed/refractory (R/R) B-cell neoplasms together with circumstances with Ph+ ALL. Given doable deeper response, extra sufferers have been anticipated to achieve optimum minimal residual illness (MRD) response.
An alternate technique, duplex droplet digital PCR (ddPCR) with excessive sensitivity was established, which may present absolute quantification of MRD with out the necessity for calibration curves. Right here, we retrospectively collected 95 bone marrow samples from 10 sufferers with R/R Ph+, who acquired 19/22 CAR-T-cell cocktail remedy. Notably, sequential molecular remission for greater than three months (SMR3), a major indicator primarily based on ddPCR after CAR-T infusion was established, which was outlined as a sequential molecular remission for not <three months with damaging MRD. On this cohort, no recurrence was noticed in six sufferers reaching SMR3, the place 4 of whom accepted allogeneic hematopoietic stem cell transplantation (allo-HSCT) after CAR-T cell routine.
Sadly, the opposite 4 sufferers who didn’t attain SMR3 relapsed, and didn’t obtain further particular therapy besides CAR-T routine. To sum up, ddPCR could also be another, particularly when nucleic acid was inadequate in medical apply. No achievement of SMR3 could be an early warning of potential relapse after CAR-T and indicating the initiation of different therapies together with allo-HSCT.
anti-GFP VHH antibody (Alexa Fluor® 647) |
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JOT0001-3-5 | Jotbody | 5 | EUR 121.77 |
Description: Conjugated VHH single domain antibody |
anti-GFP VHH antibody (Alexa Fluor® 647) |
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JOT0001-3-50 | Jotbody | 50 | EUR 340.96 |
Description: Conjugated VHH single domain antibody |
anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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JOT0002-5-1 | Jotbody | each | Ask for price |
anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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JOT0002-5-2-15 | Jotbody | 15 | EUR 221.92 |
Description: Primary VHH single domain antibody |
anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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JOT0002-5-2-5 | Jotbody | 5 | EUR 121.77 |
Description: Primary VHH single domain antibody |
anti-PD-L1 VHH antibody (Alexa Fluor® 647) |
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JOT0002-5-2-50 | Jotbody | 50 | EUR 340.96 |
Description: Primary VHH single domain antibody |
Alexa Fluor® 647 anti-human IL-1β Antibody |
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E16FHKIL1-100 | EnoGene | 100Tests | EUR 945 |
Description: Available in various conjugation types. |
SAM FCM (Alexa Fluor 647) |
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abx098902-100tests | Abbexa | 100 tests | EUR 1479.6 |
Alexa Fluor® 647 Streptavidin |
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E16FXP040K | EnoGene | 100 μg | EUR 758.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-rat CD3 |
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E16FRK003-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-rat CD8a |
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E16FRK008a-100U | EnoGene | 100 μg | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-rat CD43 |
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E16FRK043-100U | EnoGene | 100 μg | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-rat CD45 |
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E16FRK045-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-rat CD45 |
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E16FRK045-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-rat CD86 |
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E16FRK086-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 rat IgG2a |
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E16FRCK002-100U | EnoGene | 0.1 mg | EUR 628.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD3 |
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E16FMK003-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD4 |
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E16FMK004-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD5 |
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E16FMK005-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD5 |
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E16FMK005-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD9 |
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E16FMK009-025U | EnoGene | 25 μg | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD9 |
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E16FMK009-100U | EnoGene | 100 μg | EUR 931.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse IgD |
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E16SDK501-025U | EnoGene | 25 μg | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse IgD |
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E16SDK501-100U | EnoGene | 100 μg | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD3 |
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E16FHK003-025 | EnoGene | 25 tests | EUR 288.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD3 |
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E16FHK003-050 | EnoGene | 50 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD3 |
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E16FHK003-100 | EnoGene | 100 tests | EUR 682.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD4 |
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E16FHK004-025 | EnoGene | 25 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD4 |
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E16FHK004-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD5 |
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E16FHK005-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD8 |
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E16FHK008-025 | EnoGene | 25 tests | EUR 288.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD8 |
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E16FHK008-050 | EnoGene | 50 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD8 |
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E16FHK008-100 | EnoGene | 100 tests | EUR 682.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse IgM |
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E16SMK501-025U | EnoGene | 25 μg | EUR 325 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse IgM |
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E16SMK501-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 mouse IgG1 |
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E16FMCK001-100 | EnoGene | 100 tests | EUR 736.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD3e |
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E16FMK003e-025U | EnoGene | 25 μg | EUR 362.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD8a |
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E16FMK008a-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD18 |
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E16FMK018-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD19 |
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E16FMK019-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD22 |
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E16FMK022-025U | EnoGene | 25 μg | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD22 |
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E16FMK022-100U | EnoGene | 100 μg | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD23 |
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E16FMK023-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD23 |
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E16FMK023-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD24 |
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E16FMK024-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD24 |
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E16FMK024-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD25 |
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E16FMK025-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD25 |
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E16FMK025-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD31 |
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E16FMK031-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD34 |
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E16FMK034-025U | EnoGene | 25 μg | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD34 |
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E16FMK034-100U | EnoGene | 100 μg | EUR 888.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD36 |
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E16FMK036-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD39 |
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E16FMK039-025U | EnoGene | 25 μg | EUR 498.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD39 |
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E16FMK039-100U | EnoGene | 100 μg | EUR 1278.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD40 |
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E16FMK040-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD40 |
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E16FMK040-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD45 |
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E16FMK045-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD45.2 |
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E16FMK045.2-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD47 |
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E16FMK047-025U | EnoGene | 25 μg | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD47 |
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E16FMK047-100U | EnoGene | 100 μg | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD48 |
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E16FMK048-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD54 |
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E16FMK054-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD68 |
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E16FMK068-025U | EnoGene | 25 μg | EUR 541.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD68 |
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E16FMK068-100U | EnoGene | 100 μg | EUR 1278.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD69 |
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E16FMK069-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD73 |
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E16FMK073-025U | EnoGene | 25 μg | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD73 |
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E16FMK073-100U | EnoGene | 100 μg | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD80 |
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E16FMK080-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD80 |
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E16FMK080-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD83 |
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E16FMK083-100U | EnoGene | 100 μg | EUR 1191.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD86 |
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E16FMK086-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD86 |
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E16FMK086-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD90.2 |
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E16FMK090.2-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-rat CD45RC |
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E16FRK045RC-100U | EnoGene | 100 μg | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD1a |
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E16FHK001a-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD1c |
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E16FHK001c-100 | EnoGene | 100 tests | EUR 1083.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD8a |
|||
E16FHK008a-025 | EnoGene | 25 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD8a |
|||
E16FHK008a-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD14 |
|||
E16FHK0142-025 | EnoGene | 25 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD14 |
|||
E16FHK0142-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD16 |
|||
E16FHK016-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD16 |
|||
E16FHK016-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD19 |
|||
E16FHK019-025 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD19 |
|||
E16FHK019-100 | EnoGene | 25 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD20 |
|||
E16FHK020-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD24 |
|||
E16FHK024-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD24 |
|||
E16FHK024-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD25 |
|||
E16FHK025-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD25 |
|||
E16FHK025-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD27 |
|||
E16FHK027-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD29 |
|||
E16FHK029-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD29 |
|||
E16FHK029-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD31 |
|||
E16FHK031-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD31 |
|||
E16FHK031-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD32 |
|||
E16FHK032-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD34 |
|||
E16FHK034-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD34 |
|||
E16FHK034-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD38 |
|||
E16FHK038-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD40 |
|||
E16FHK040-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD40 |
|||
E16FHK040-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD45 |
|||
E16FHK045-025 | EnoGene | 25 tests | EUR 288.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD45 |
|||
E16FHK045-050 | EnoGene | 50 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD45 |
|||
E16FHK045-100 | EnoGene | 100 tests | EUR 682.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD54 |
|||
E16FHK054-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD57 |
|||
E16FHK057-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD61 |
|||
E16FHK061-025 | EnoGene | 25 tests | EUR 520 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD61 |
|||
E16FHK061-100 | EnoGene | 100 tests | EUR 1083.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD63 |
|||
E16FHK063-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD63 |
|||
E16FHK063-100 | EnoGene | 100 tests | EUR 1170 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD64 |
|||
E16FHK064-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD69 |
|||
E16FHK069-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD80 |
|||
E16FHK080-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD80 |
|||
E16FHK080-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD82 |
|||
E16FHK082-100 | EnoGene | 100 tests | EUR 1148.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD83 |
|||
E16FHK083-100 | EnoGene | 100 tests | EUR 1083.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD86 |
|||
E16FHK086-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD86 |
|||
E16FHK086-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD94 |
|||
E16FHK094-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse IgG1 |
|||
E16SHK501-025U | EnoGene | 25 μg | EUR 325 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse IgG1 |
|||
E16SHK501-100U | EnoGene | 100 μg | EUR 715 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD11a |
|||
E16FMK011a-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD11a |
|||
E16FMK011a-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD11c |
|||
E16FMK011c-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD11c |
|||
E16FMK011c-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD49b |
|||
E16FMK049b-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD49d |
|||
E16FMK049d-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD49d |
|||
E16FMK049d-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD49e |
|||
E16FMK049e-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD62L |
|||
E16FMK062L-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD102 |
|||
E16FMK102-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD103 |
|||
E16FMK103-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD104 |
|||
E16FMK104-025U | EnoGene | 25 μg | EUR 585 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD104 |
|||
E16FMK104-100U | EnoGene | 100 μg | EUR 1365 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD106 |
|||
E16FMK106-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse CD106 |
|||
E16FMK106-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD11a |
|||
E16FHK011a-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD11b |
|||
E16FHK011b-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD11b |
|||
E16FHK011b-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD11b |
|||
E16FHK011b-100U | EnoGene | 100 μg | EUR 845 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD11c |
|||
E16FHK011c-025 | EnoGene | 25 tests | EUR 411.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD11c |
|||
E16FHK011c-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD11c |
|||
E16FHK011c-100U | EnoGene | 100 μg | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD49a |
|||
E16FHK049a-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD62L |
|||
E16FHK062L-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD66b |
|||
E16FHK066b-025 | EnoGene | 25 tests | EUR 541.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD66b |
|||
E16FHK066b-100 | EnoGene | 100 tests | EUR 1191.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD105 |
|||
E16FHK105-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD123 |
|||
E16FHK123-025 | EnoGene | 25 tests | EUR 563.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD123 |
|||
E16FHK123-100 | EnoGene | 100 tests | EUR 1148.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD146 |
|||
E16FHK146-025 | EnoGene | 25 tests | EUR 476.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD146 |
|||
E16FHK146-100 | EnoGene | 100 tests | EUR 1061.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD147 |
|||
E16FHK147-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD147 |
|||
E16FHK147-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD154 |
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E16FHK154-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD160 |
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E16FHK160-025 | EnoGene | 25 tests | EUR 650 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD160 |
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E16FHK160-100 | EnoGene | 100 tests | EUR 1408.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD161 |
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E16FHK161-025 | EnoGene | 25 tests | EUR 541.67 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD161 |
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E16FHK161-100 | EnoGene | 100 tests | EUR 1278.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD163 |
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E16FHK163-025 | EnoGene | 25 tests | EUR 433.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD163 |
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E16FHK163-100 | EnoGene | 100 tests | EUR 975 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse IgG2a |
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E16SKK501-025U | EnoGene | 25 μg | EUR 325 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-mouse IgG2a |
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E16SKK501-100U | EnoGene | 100 μg | EUR 715 |
Description: Available in various conjugation types. |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 647 |
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nAP-20508 | Angio Proteomie | 500ug | EUR 183.6 |
Alexa Fluor® 647 anti-mouse CD45RB |
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E16FMK045RB-025U | EnoGene | 25 μg | EUR 368.33 |
Description: Available in various conjugation types. |
Alexa Fluor® 647 anti-human CD45RA |
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E16FHK0453-025 | EnoGene | 25 tests | EUR 455 |
Description: Available in various conjugation types. |
Ilościowe wykrywanie i monitorowanie Colletotrichum siamense na drzewach kauczukowych przy użyciu PCR w czasie rzeczywistym
Colletotrichum siamense is without doubt one of the most necessary pathogens of rubber timber in Asia. The right detection and quantification of C. siamense populations in rubber timber are of significance for monitoring the epidemics of the illness. On this research, we developed an ITS-based real-time PCR technique to effectively detect C. siamense infecting rubber tree, which reliably detected as little as 100 fg genomic DNA, 100 copies of goal DNA and 20 conidia.
The actual-time PCR protocol acknowledged all C. siamense isolates collected from three provinces in China, whereas no amplification was noticed with rubber tree and its different pathogens. Detection and quantification of C. siamense have been carried out in artificially and naturally contaminated rubber leaves. We may nonetheless detect C. siamense in plant mixes of which solely 0.0001% of the tissue contaminated. An accumulation of C. siamense DNA was noticed throughout the entire an infection course of in any respect three leaf phenological levels, suggesting the real-time PCR technique can be utilized to watch C. siamense improvement in rubber timber. Lastly, the tactic allowed the detection of C. siamense in naturally contaminated and symptomless leaves of rubber timber within the fields. In contrast with earlier detection strategies, the real-time PCR technique is extra particular and extra delicate, and might be of nice use for research aiming to achieve a greater understanding of the epidemiology of Colletotrichum leaf illness, in addition to the prediction of illness danger and the management proposal.
Ilościowy pomiar liczby kopii transpozonu przy użyciu cyfrowej PCR kropli
Spontaneous proliferation of transposable parts contributes to genetic range at various ranges reminiscent of somatic mosaicism, genetic divergence in inhabitants, and genome evolution. Such genetic range is important for vegetation’ adaptation to altering atmosphere and serves as a useful useful resource for crop enchancment. Subsequently, measuring the copy quantity variation of transposable parts with precision and effectivity is necessary to grasp the extent of their proliferation.
Droplet Digital PCR (ddPCR) is an correct and delicate method that permits measurement of copy quantity variation of a transposon. Briefly, genomic DNA is extracted, digested, and partitioned into 1000’s of nanoliter-scale droplets. The TaqMan real-time PCR adopted by the end-point fluorescence detection allows the quantitative measurement of copy variety of template DNAs. Right here on this chapter, we describe the step-by-step process of ddPCR utilizing EVADE retrotransposon of Arabidopsis for example.
Porównanie wyników tomografii komputerowej (CT) z RT-PCR w diagnostyce COVID-19 u dzieci
polymerase chain response (RT-PCR) check ends in kids with possible or definitive prognosis of coronavirus illness 2019 (COVID-19).
Strategies: On this retrospective archive research, pediatric sufferers who have been adopted up within the hospital with a doable or definitive prognosis of COVID-19 and who had chest CT at presentation have been included. CT scan photos of the sufferers have been reinterpreted by a pediatric radiologist and in contrast with their RT-PCR check outcomes.
Outcomes: Of the whole of 89 sufferers, 33 had damaging and 56 had constructive RT-PCR exams. The presence of pulmonary lesions and consolidation was statistically considerably greater within the RT-PCR damaging group than within the RT-PCR constructive group (p = 0.037 and 0.001, respectively). Lobe involvement of 0%-25% was greater within the RT-PCR constructive group (p = 0.001), and lobe involvements of 25%-50% and 50%-75% have been considerably greater within the RT-PCR damaging group (p = 0.001 and 0.005, respectively). Central and perihilar involvement was discovered to be statistically important in the RT-PCR damaging group (p = 0.008 and 0.005, respectively).
Conclusion: Chest CT findings could present some clues in predicting RT-PCR positivity in kids with a possible prognosis of COVID-19. Lobe involvement proportion of as much as 25% is a discovering in favor of sufferers with constructive RT-PCR check, whereas 25%-75% lobe involvement, central and perihilar involvement, and consolidation might be interpreted in favor of sufferers with damaging RT-PCR check.
Integracja przygotowania próbek mikroprzepływowych z detekcją PCR w celu zbadania skutków jednoczesnej separacji inhibitora DNA i wymiany roztworu DNA
On this paper, we utilized a curved-channel microfluidic system to separate DNA from PCR-inhibitor-containing water and concurrently wash them into clear water for detection utilizing a conveyable PCR thermocycler. Environmental DNA (eDNA) sampling has turn into an efficient surveying method for detecting uncommon organisms. Nonetheless, low focus eDNA molecules could also be masked by PCR inhibitors throughout amplification and detection, growing the chance of false negatives. Subsequently, applied sciences for on-site DNA separation and washing are urgently wanted. Our system consisted of a half-circle microchannel with a DNA-inhibitor pattern inlet, a clear buffer inlet, and a number of retailers. Through the use of the flow-induced inertial forces, 10 μm DNA-conjugated microparticles have been targeted on the inner-wall of the curved microchannel whereas separation from 1 μm inhibitor-conjugated microparticles and DNA washing have been achieved concurrently with the Dean circulation.
We achieved singleplex focusing, isolation and washing of 10 μm particles at an effectivity of 94.5 ± 2.0%. In duplex experiments with 1 μm and 10 μm particles, bigger particles have been washed with an effectivity of 92.1 ± 1.6% and a purity of 79 ± 2%. By surface-functionalizing the microparticles with affinity teams towards Atlantic salmon DNA and humic acid (HA), and processing samples of varied concentrations in our system, we achieved an efficient purification and detection of DNA molecules utilizing the transportable PCR thermocycler. Our technique considerably decreased PCR quantitation cycles from Cq > 38 to Cq = 30.35 ± 0.5, which confirmed enhancement of PCR amplification. The proposed system takes a promising step ahead in pattern preparation in the direction of an built-in system that can be utilized for simultaneous purification and resolution alternate of DNA in point-of-need environmental monitoring functions.